LOCUS       40924_959        8923 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GAANTRY B donor tdTom, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8923)
  AUTHORS   Collier R, Thomson JG, Thilmony R.
  TITLE     GAANTRY, a versatile and robust Agrobacterium-based gene stacking 
            system generates high quality transgenic plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8923)
  AUTHORS   Thilmony R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2017) Crop Improvement and Genetics research Unit,
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   3  (bases 1 to 8923)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8923)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-DEC-2017) Crop Improvement and Genetics research Unit, 
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(38..212)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     regulatory      complement(218..446)
                     /label=nos terminator
                     /note="nos terminator"
                     /regulatory_class="terminator"
     CDS             complement(490..1917)
                     /label=tdTomato
                     /note="tandem dimeric (pseudo-monomeric) derivative of
                     DsRed (Shaner et al., 2004)"
     promoter        complement(2032..2708)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    2937..2991
                     /label=TP901 attB
                     /note="TP901 attB"
     misc_feature    3013..3037
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        3196..3224
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3413..3943
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     misc_feature    4022..4127
                     /label=ParA MRS
                     /note="ParA MRS"
     primer_bind     complement(4150..4166)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4174..4190)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4198..4228)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4243..4264)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4497..4942
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             4943..6361
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
     rep_origin      complement(6462..7050)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7224..8081)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8082..8186)
                     /label=AmpR promoter
     rep_origin      complement(8212..8667)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     8809..8825
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8856..8911
                     /label=A118 attB
                     /note="A118 attB"
ORIGIN
        1 ttttaatgta ctgaattaac gccgaattaa ttcgggggat ctggatttta gtactggatt
       61 ttggttttag gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact
      121 aagggtttct tatatgctca acacatgagc gaaaccctat aggaacccta attcccttat
      181 ctgggaacta ctcacacatt attatggaga aactcgacgc gcgataattt atcctagttt
      241 gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata
      301 aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa
      361 ttcaacagaa attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa
      421 ctttattgcc aaatgtttga acgatcgggg aaattcgagc tggtcaccac tagtttacaa
      481 ctcgtccttt ttgtaaagct cgtccattcc gtatagaaaa aggtggtgac gtccttctga
      541 tctttcatat tgctccacga tagtgtagtc ttcgttatga gaggtaatat cgagtttggt
      601 atcgacgtag tagtagccag gtagctgcac gggctttttc gccatatata tcgtcttaaa
      661 ttccaccaga tagtgccccc cgtccttcag tttaagcgcc tggtgtatct cccctttcaa
      721 aaccccgtcc ctcgggtaca gcctctccgt gctggcctcc cagcccatag tttttttctg
      781 catcaccggg ccgtccgggg gaaaattcgt acccctcatt ttgactttgt atatcaatgt
      841 gccatcctgc agactcgaat cttgtgtaac agtaaccagt ccaccatcct cgaaattcat
      901 aacccgctcc catttaaacc cctccgggaa agacaatttc ttataatcgg ggatatcagc
      961 cggatgttta acgtaggcct tggagccata catgaactga ggagagagaa tgtcccaggc
     1021 aaaaggaagc ggcccacctt tggtgacttt tagtttcgcg gtttgtgtcc cctcatatgg
     1081 tcggccctcg ccttctccct caatttcaaa ttcgtgccca ttcatgctgc cctccatacg
     1141 gactttaaac cgcatgaact ctttgatcac cgccatgttg ttgtcctccg agcttgccgt
     1201 gccgctggaa cccgagcccg tggaacccgt gccatgtccg aggaatagat ggtgacgacc
     1261 ctcactgcgc tcatactgtt ccactatggt gtaatcttcg ttgtgggacg ttatatccag
     1321 ttttgtgtcc acatagtaat atcccggcaa ctggacgggt ttcttggcca tatatatagt
     1381 tttgaactca accaggtagt ggccgccgtc ctttagtttc agtgcctgat ggatttcgcc
     1441 tttgagcact ccgtctctcg ggtaaagtct ctcggtggat gcctcccatc ccattgtttt
     1501 tttctgcata acagggccat caggggggaa atttgttccc ctcatcttca ccttgtaaat
     1561 tagggtgccg tcttgaagtg aggagtcttg agtcacagtg acgagccctc cgtcttcaaa
     1621 attcatcact ctttcccatt taaacccctc cgggaacgac agctttttgt aatcagggat
     1681 gtcggcgggg tgcttaacgt atgccttgga accgtacata aattgtggac tcagaatgtc
     1741 ccaggcgaag ggcagtgggc cgcccttcgt cactttcagt ttcgccgtct gggtgccctc
     1801 gtatggtcta ccttcgcctt caccttcgat ctcgaactca tggccgttca tactgccctc
     1861 cattctcact ttgaacctca tgaactcttt aatcacctct tcgcccttgg acaccatgtc
     1921 cgcgctggaa agtgagagga gaagcgagaa gatgaagaaa aggaaaagat tagtcttcac
     1981 catgtttaat taactcgaga gagatagatt tgtagagaga gactggtgat ttcagcgtgt
     2041 cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga
     2101 ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt
     2161 ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
     2221 actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta
     2281 ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa
     2341 tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc
     2401 tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttcac
     2461 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
     2521 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc
     2581 ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga
     2641 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa
     2701 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga
     2761 gagtgtcgtg ctccaccatg ttggcaagct gctctagcca atacgcaaac cgcggccgct
     2821 tttcctaggt tttaacgttt tttggatcct tttgtttaaa cttttaagct ttttttctag
     2881 attttaacgt tttttggatc cttttgttta aacttttggc gcgccgttta aacaaactga
     2941 taattgccaa cacaattaac atctcaatca aggtaaatgc tttttgcttt taaagtttaa
     3001 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta
     3061 gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
     3121 atgccaacca cagggttccc ctcgggagaa acgtacggac gcacaccgtg gaaacggatg
     3181 aaggcacgaa cccagttgac ataagcctgt tcggttcgta aactgtaatg caagtagcgt
     3241 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     3301 gcggttttca tggcttgtta tgactgtttt tttgtacagt ctatgcctcg ggcatccaag
     3361 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     3421 cagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttag gtggctcaag
     3481 tatgggcatc attcgcacat gtaggctcgg ccctgaccaa gtcaaatcca tgcgggctgc
     3541 tcttgatctt ttcggtcgtg agttcggaga cgtagccacc tactcccaac atcagccgga
     3601 ctccgattac ctcgggaact tgctccgtag taagacattc atcgcgcttg ctgccttcga
     3661 ccaagaagcg gttgttggcg ctctcgcggc ttacgttctg cccaagtttg agcagccgcg
     3721 tagtgagatc tatatctatg atctcgcagt ctccggagag caccggaggc agggcattgc
     3781 caccgcgctc atcaatctcc tcaagcatga ggccaacgcg cttggtgctt atgtgatcta
     3841 cgtgcaagca gattacggtg acgatcccgc agtggctctc tatacaaagt tgggcatacg
     3901 ggaagaagtg atgcactttg atatcgaccc aagtaccgcc acctaacaat tcgttcaagc
     3961 cgagatcggc ttcccggccg cggagttgtt cggtaaattg tcacaacacc gccgtacgaa
     4021 aggtcaaatt gggtataccc atttgggcct agtctagccg gcatggcgca ttacagcaat
     4081 acgcaattta aatgcgccta gcgcattttc ccgaccttaa tgcgcctaaa cgcgcttggc
     4141 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     4201 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     4261 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     4321 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     4381 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     4441 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatggcaca
     4501 tatacctgcc gttcactatt atttagtgaa atgagatatt atgatatttt ctgaattgtg
     4561 attaaaaagg caactttatg cccatgcaac agaaactata aaaaatacag agaatgaaaa
     4621 gaaacagata gattttttag ttctttaggc ccgtagtctg caaatccttt tatgattttc
     4681 tatcaaacaa aagaggaaaa tagaccagtt gcaatccaaa cgagagtcta atagaatgag
     4741 gtcgaaaagt aaatcgcgcg ggtttgttac tgataaagca ggcaagacct aaaatgtgta
     4801 aagggcaaag tgtatacttt ggcgtcaccc cttacatatt ttaggtcttt ttttattgtg
     4861 cgtaactaac ttgccatctt caaacaggag ggctggaaga agcagaccgc taacacagta
     4921 cataaaaaag gagacatgaa cgatgaacat caaaaagttt gcaaaacaag caacagtatt
     4981 aacctttact accgcactgc tggcaggagg cgcaactcaa gcgtttgcga aagaaacgaa
     5041 ccaaaagcca tataaggaaa catacggcat ttcccatatt acacgccatg atatgctgca
     5101 aatccctgaa cagcaaaaaa atgaaaaata tcaagttcct gagttcgatt cgtccacaat
     5161 taaaaatatc tcttctgcaa aaggtctgga cgtttgggac agctggccat tacaaaacgc
     5221 tgacggcact gtcgcaaact atcacggcta ccacatcgtc tttgcattag ccggagatcc
     5281 taaaaatgcg gatgacacct cgatttatat gttctatcaa aaagtcggcg aaacttctat
     5341 tgacagctgg aaaaacgctg gccgcgtctt taaagacagc gacaaattcg atgcaaatga
     5401 ttctatccta aaagaccaaa cacaagaatg gtcaggttca gccacattta catctgacgg
     5461 aaaaatccgt ttattctaca ctgatttctc cggtaaacat tacggcaaac aaacactgac
     5521 aactgcacaa gtcaacgtat cagcatcaga cagctctttg aacatcaacg gtgtagagga
     5581 ctataaatca atctttgacg gtgacggaaa aacgtatcaa aatgtccagc agttcatcga
     5641 cgaaggcaac tacagctcag gcgacaacca tacgctgaga gatcctcact acgtagaaga
     5701 taaaggccac aaatacttag tatttgaagc aaacactgga actgaagatg gctaccaagg
     5761 cgaagaatct ttatttaaca aagcatacta tggcaaaagc acatcattct tccgtcaaga
     5821 aagtcaaaaa cttctgcaaa gcgataaaaa acgcacggct gagttagcaa acggcgctct
     5881 cggtatgatt gagctaaacg atgattacac actgaaaaaa gtgatgaaac cgctgattgc
     5941 atctaacaca gtaacagatg aaattgaacg cgcgaacgtc tttaaaatga acggcaaatg
     6001 gtatctgttc actgactccc gcggatcaaa aatgacgatt gacggcatta cgtctaacga
     6061 tatttacatg cttggttatg tttctaattc tttaactggc ccatacaagc cgctgaacaa
     6121 aactggcctt gtgttaaaaa tggatcttga tcctaacgat gtaaccttta cttactcaca
     6181 cttcgctgta cctcaagcga aaggaaacaa tgtcgtgatt acaagctata tgacaaacag
     6241 aggattctac gcagacaaac aatcaacgtt tgcgccgagc ttcctgctga acatcaaagg
     6301 caagaaaaca tctgttgtca aagacagcat ccttgaacaa ggacaattaa cagtcaacaa
     6361 ataaaaacgc aaaagaaaat gccgatatgc tatcggcatt acatgtgagc aaaaggccag
     6421 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6481 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6541 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6601 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6661 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6721 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6781 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6841 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6901 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6961 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7021 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7081 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7141 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7201 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7261 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7321 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7381 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7441 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7501 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7561 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7621 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7681 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7741 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7801 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7861 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7921 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7981 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8041 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8101 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8161 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
     8221 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     8281 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     8341 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     8401 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     8461 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     8521 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     8581 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     8641 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
     8701 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     8761 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     8821 ccagtgagcg cgttaacaaa agtactttta gatctgtaac tttttcggat caagctatga
     8881 aggacgcaaa gagggaacta aacacttaat tacgcgtacg cgt
//
